Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Brilliant Violet 650, eBioscience™

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 5 µL (1.0 µg)/test
Publications: -

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha exhibits cytolytic activity against various tumor cells and plays a key role in immune regulation. It exists as soluble dimers/trimers and as a 26 kDa membrane-bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported:
Validated for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-diluted and tested using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended usage is 5 µL (1.0 µg) per 100 µL test volume. Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm and is designed for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple Brilliant Violet™ or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
BV650 is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) with minimal loss of brightness. BV650 is not compatible with methanol-based fixation.

Excitation/Emission: 407 nm / 649 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine of the TNF superfamily, secreted mainly by macrophages. It binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, regulating immune response, cell proliferation, apoptosis, and metabolism. TNF alpha appears as a 17 kDa band under non-reducing SDS-PAGE conditions and has been linked to tumor necrosis, immune response, and various diseases including autoimmune disorders and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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