Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Brilliant Violet 650, eBioscience
마우스 IFN-γ 검출용 중화 모노클론 항체로 Brilliant Violet 650 형광체가 결합되어 있습니다. Flow Cytometry용으로 검증되었으며, 높은 특이성과 안정적인 형광 신호를 제공합니다. 4°C 암소 보관, 메탄올 고정 불가.
- 카탈로그번호
- 416-7311-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Brilliant Violet 650, eBioscience
Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Brilliant Violet 650, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet™ 650, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | XMG1.2 |
| Conjugate | Brilliant Violet™ 650 |
| Excitation / Emission Max | 407 / 646 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925707 |
Additional Formats
- Unconjugated
- Alexa Fluor 488, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 711, 786
- eFluor 450, 660
- FITC
- Functional Grade
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The XMG1.2 antibody reacts with mouse interferon (IFN) gamma and acts as a neutralizing antibody. IFN gamma is a 20 kDa cytokine produced by activated T, B, and NK cells, exhibiting antiviral and antiparasitic activity. It synergizes with TNF alpha to inhibit cell proliferation and modulates immune responses across various cell types expressing IFN gamma receptors. Glycosylation does not affect its biological activity.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Verified using mouse splenocytes and the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended usage ≤1.0 µg/test (100 µL final volume).
Brilliant Violet™ 650 (BV650) emits at 649 nm and is suitable for cytometers equipped with a 405 nm violet laser. Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). Not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 649 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation and immune interferon produced mainly by T-lymphocytes and NK cells. It is a dimeric glycoprotein (~45 kDa) with antiviral, antiproliferative, and immunomodulatory functions. Human IFN gamma does not cross-react with mouse IFN gamma. It activates macrophages, induces MHC expression, and enhances B-cell immunoglobulin secretion via the JAK-STAT pathway. Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.