Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 711, 786
• eFluor 450, 660
• FITC
• Functional Grade
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The XMG1.2 antibody reacts with mouse interferon (IFN) gamma and acts as a neutralizing antibody. IFN gamma is a 20 kDa cytokine produced by activated T, B, and NK cells, exhibiting antiviral and antiparasitic activity. It synergizes with TNF alpha to inhibit cell proliferation and modulates immune responses across various cell types expressing IFN gamma receptors. Glycosylation does not affect its biological activity.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Verified using mouse splenocytes and the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended usage ≤1.0 µg/test (100 µL final volume).

Brilliant Violet™ 650 (BV650) emits at 649 nm and is suitable for cytometers equipped with a 405 nm violet laser. Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). Not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 649 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation and immune interferon produced mainly by T-lymphocytes and NK cells. It is a dimeric glycoprotein (~45 kDa) with antiviral, antiproliferative, and immunomodulatory functions. Human IFN gamma does not cross-react with mouse IFN gamma. It activates macrophages, induces MHC expression, and enhances B-cell immunoglobulin secretion via the JAK-STAT pathway. Mutations in the IFN gamma gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures.

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