Thermo Fisher Scientific Phospho-Paxillin (Tyr118) Polyclonal Antibody
인산화된 Paxillin(Tyr118)을 특이적으로 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 44722G
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-Paxillin (Tyr118) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 47 publications |
| Immunohistochemistry (IHC) | Assay-dependent | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 publications |
| Immunocytochemistry (ICC/IF) | - | 37 publications |
| Immunoprecipitation (IP) | - | 1 publication |
| in situ PLA (PLA) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Chicken, Dog, Human, Mouse, Pig, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from the region of human paxillin containing tyrosine 118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533733 |
Product Specific Information
This antibody was negatively preadsorbed using:
- A non-phosphopeptide corresponding to the phosphorylation site to remove reactivity with non-phosphorylated paxillin.
- A generic tyrosine-phosphorylated peptide to remove reactivity with phosphotyrosine irrespective of sequence.
The final antibody was affinity-purified using a paxillin-derived peptide phosphorylated at tyrosine 118.
Positive controls for Western blotting:
- Normal mouse mammary gland (NMuMG) cells
- NMuMG cells transfected with EGFP-tagged paxillin ± TGFβ
- Human epidermoid carcinoma cells (A431) ± EGF
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as Src, FAK, and p210BRC/ABL. Its tyrosine phosphorylation is influenced by changes in cell-cell adhesion. Paxillin interacts with FAK and Crk via its SH2 domain, independently of the extracellular matrix. Although initially identified in fibroblasts, paxillin phosphorylation also plays a role in neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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