Thermo Fisher Scientific Phospho-MSK1 (Thr581) Recombinant Rabbit Monoclonal Antibody (MSK1T581-A5)
인산화된 MSK1(Thr581)을 특이적으로 인식하는 재조합 토끼 단클론 항체로, Western blot 및 유세포 분석에 적합합니다. 높은 특이성과 재현성을 제공하며, 로트 간 일관성과 동물 유래 성분이 없는 포뮬레이션을 특징으로 합니다.
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Applications
Western Blot (WB)
- Assay-dependent
Flow Cytometry (Flow)
- 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MSK1T581-A5 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr581 of human phospho MSK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745070 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
RPS6KA5 (MSK1) is a serine/threonine protein kinase involved in regulating various cellular processes. It mediates mitogen- or stress-induced phosphorylation of transcription factors such as CREB1 and ATF1, and regulates RELA, STAT3, and ETV1/ER81.
MSK1 contributes to gene activation via histone phosphorylation and plays a key role in inflammatory gene regulation. It phosphorylates CREB1 and ATF1 in response to stimuli like UV-C irradiation, EGF, and anisomycin, and modulates RELA transcriptional activity in response to TNF and glucocorticoids. In skeletal myoblasts, it is required for RELA phosphorylation at Ser-276 during oxidative stress, and in erythropoietin-stimulated cells, it mediates STAT3 Ser-727 phosphorylation, influencing transcriptional regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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