Thermo Fisher Scientific Phospho-MSK1 (Thr581) Recombinant Rabbit Monoclonal Antibody (MSK1T581-A5)

Applications

Western Blot (WB)

• Assay-dependent

Flow Cytometry (Flow)

• 1–1,000 ng/mL

Product Specifications

Additional Formats

• APC
• FITC
• PE
• Request custom conjugation

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates.
Advantages include:

• Enhanced specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to the larger rabbit immune repertoire

Target Information

RPS6KA5 (MSK1) is a serine/threonine protein kinase involved in regulating various cellular processes. It mediates mitogen- or stress-induced phosphorylation of transcription factors such as CREB1 and ATF1, and regulates RELA, STAT3, and ETV1/ER81.
MSK1 contributes to gene activation via histone phosphorylation and plays a key role in inflammatory gene regulation. It phosphorylates CREB1 and ATF1 in response to stimuli like UV-C irradiation, EGF, and anisomycin, and modulates RELA transcriptional activity in response to TNF and glucocorticoids. In skeletal myoblasts, it is required for RELA phosphorylation at Ser-276 during oxidative stress, and in erythropoietin-stimulated cells, it mediates STAT3 Ser-727 phosphorylation, influencing transcriptional regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.