Thermo Fisher Scientific Phospho-p53 (Ser15) Recombinant Rabbit Monoclonal Antibody (P53S15-1C11), FITC
FITC 형광 표지된 p53 인산화(Ser15) 특이 재조합 토끼 단클론 항체로서, 인간 및 생쥐 시료에 반응합니다. Flow cytometry에 적합하며 높은 특이성과 일관된 로트 간 재현성을 제공합니다. 암 연구 및 세포 스트레스 반응 분석에 활용됩니다.
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Thermo Fisher Scientific Phospho-p53 (Ser15) Recombinant Rabbit Monoclonal Antibody (P53S15-1C11), FITC
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL/1x10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | P53S15-1C11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser15 of human phospho p53 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745068 |
Additional Formats
- Unconjugated
- APC
- PE
- Custom conjugation available upon request
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation.
Key Characteristics:
- Composed of N-terminal transactivation, central DNA-binding, oligomerization, and C-terminal regulatory domains
- Phosphorylation at Ser15 is crucial for activation and stabilization
- Involved in tumor suppression, aging, oxidative stress response, and learning/memory processes
- Mutations lead to loss of DNA binding and tumor suppressor activity
- Alterations in TP53 gene are associated with various cancers and Li-Fraumeni syndrome
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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