Thermo Fisher Scientific p53 Monoclonal Antibody (OTI2F7), TrueMAB

Thermo Fisher Scientific p53 Monoclonal Antibody (OTI2F7), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain an antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 activation leads to transcription of genes such as p21waf1, contributing to G1 or G2/M cell cycle arrest.

Structurally, p53 consists of:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization. p53 helps prevent proliferation of damaged cells and acts as an anticancer factor. Alterations or mutations in the TP53 gene are common in human cancers and lead to loss of tumor suppressor function. Germline mutations are associated with cancer-prone syndromes such as Li-Fraumeni syndrome.

Notice

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.