Thermo Fisher Scientific Met Recombinant Rabbit Monoclonal Antibody (BLR283L)
Recombinant rabbit monoclonal antibody (BLR283L) targeting human MET protein. Validated for WB, IHC, ICC/IF, Flow, and IP applications. High specificity and purity for research use. Supplied as liquid form, 1 mg/mL concentration, stored at 4°C.
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Thermo Fisher Scientific Met Recombinant Rabbit Monoclonal Antibody (BLR283L)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 0.5 µL / 10^6 cells |
| Immunoprecipitation (IP) | 6 µL / mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR283L |
| Immunogen | Peptide representing a region between residue 1340 and the C-terminus (residue 1390) of Human Hepatocyte Growth Factor Receptor (MET, c-Met) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunocytochemistry, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
MET (c-Met) is a receptor-like tyrosine kinase linked to various human malignancies. Upon ligand activation, MET interacts with PI3K subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3, leading to activation of signaling cascades such as RAS-ERK, PI3K-AKT, and PLCγ-PKC.
MET is involved in embryonic development (gastrulation, muscle and neuron formation, angiogenesis, kidney development) and adult processes (wound healing, organ regeneration, tissue remodeling). Dysregulation of MET is associated with cancers including gastric, renal, and breast, making it a significant target for cancer therapeutics and diagnostics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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