Thermo Fisher Scientific Troponin I Recombinant Rabbit Monoclonal Antibody (BLR280L)
Troponin I에 특이적인 재조합 토끼 단클론 항체로, Western blot과 IHC(파라핀 절편)에 적합. 인간과 생쥐 시료 반응성. 고순도 정제 및 BBS buffer(pH 8.2) 보존. 심근 손상 관련 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR280L |
| Immunogen | Peptide representing a region between residue 0 and 50 of Human Troponin I (cardiac muscle) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 250 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Cardiac Troponin I (also known as cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that functions as part of a complex with troponin C (TnC) and troponin T (TnT).
The TnT subunit binds to tropomyosin to form a troponin-tropomyosin complex anchored by TnI binding to actin within muscle thin filaments.
Upon calcium binding to TnC, conformational changes release the inhibitory region of TnI from actin, enabling myosin attachment and muscle contraction.
Measurements of blood TnI and TnT levels serve as key diagnostic indicators of cardiac muscle damage, helping to differentiate angina from myocardial infarction.
Cardiac Troponin I has also been reported to inhibit angiogenesis both in vivo and in vitro.
Mutations in the Cardiac Troponin I gene are associated with familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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