Thermo Fisher Scientific EHD1 Recombinant Rabbit Monoclonal Antibody (5S4P9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5S4P9

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EHD1 (Q9H4M9). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EHD1,uniProtId:Q9H4M9-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:534,antigenImageFileName:MA5-42814_EHD1_Q9H4M9-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42814_EHD1_Q9H4M9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.28 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911955

Product Specific Information

Positive test controls include: HeLa, THP-1, U-251MG, Mouse liver, Mouse brain, Mouse spleen, Rat lung, Rat testis, Rat kidney. The target is usually found in the following locations: Cell membrane, Cell projection, Cytoplasmic side, Early endosome membrane, Peripheral membrane protein, Recycling endosome membrane, cilium membrane.

Immunogen sequence: MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV

Target Information

EHD1 acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. EHD1 also plays a role in myoblast fusion and is recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Further, EHD1 belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The EHD1 protein is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for the EHD1 gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.