Thermo Fisher Scientific FAK Recombinant Rabbit Monoclonal Antibody (SR46-04)
FAK 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 분석에 적합하며, HEK293 세포에서 발현된 IgG 형 항체입니다. TBS buffer에 보존되어 연구용으로 사용됩니다.
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Thermo Fisher Scientific FAK Recombinant Rabbit Monoclonal Antibody (SR46-04)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR46-04 |
| Immunogen | Synthetic peptide within human FAK (aa 700–740) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2898847 |
Product Specific Information
This product is predicted to work in Immunoprecipitation.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that serves as a substrate for Src and is a key component of integrin signaling.
FAK plays a crucial role in:
- Cell spreading, differentiation, migration, and cell death
- Regulation of the G1–S phase transition in the cell cycle
FAK has:
- A central catalytic domain
- A C-terminal tail that localizes to focal adhesions, sites where cells attach to the extracellular matrix via integrin receptors
Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin.
Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of Grb2 and c-Src PTK with FAK, resulting in activation of ERK2 MAP kinase.
In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion.
Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK at Tyr925.
Several transcript variants encoding different isoforms have been identified for the FAK gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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