Thermo Fisher Scientific 3-Methylcytosine Polyclonal Antibody

Applications

Tested Dilution

Publications

Peptide Array (Array)

1:400

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human BSA-conjugated 3-methylcytosine (3-mC).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.8 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.05% sodium azide, 0.05% ProClin 300

Storage conditions

-20°C or -80°C if preferred

RRID

AB_2610672

Target Information

3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.