Thermo Fisher Scientific 5-hydroxymethylcytosine Polyclonal Antibody

Applications

Tested Dilution

Publications

ELISA (ELISA)

1:2,800

-

Immunoprecipitation (IP)

3.5 µg

-

Peptide Array (Array)

1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human KLH-conjugated 5-hydroxymethylcytosine.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.05% ProClin 300, 0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610671

Target Information

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.