Thermo Fisher Scientific H3K17me2aK18ac Polyclonal Antibody
H3K17me2aK18ac 변형 히스톤을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA, ChIP, ChIP-Seq, Peptide Array에 사용 가능. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer에 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| ELISA | 1:28,800 |
| ChIP assay (ChIP) | 1 µg |
| ChIP-sequencing (ChIP-Seq) | Assay-dependent |
| Peptide Array (Array) | 1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to the region of human histone H3 containing the asymmetrically dimethylated R17 and the acetylated lysine 18 (H3R17me2(asym)) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.94 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.05% sodium azide, 0.05% ProClin 300 |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608319 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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