Thermo Fisher Scientific IL-4 Polyclonal Antibody, Biotin
인간 IL-4 단백질을 인식하는 Biotin 결합 토끼 Polyclonal 항체. Western blot, IHC, ELISA에 적합하며, 고순도의 정제 항체로 다양한 면역반응 분석에 활용 가능. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This purified antibody has been heated to 56°C for 30 minutes.
In ELISA and other immunoreactive assays, this antibody recognizes both native and recombinant human IL-4 in cell supernatants and certain body fluids.
A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor.
IL-4 has a broad range of actions and shares overlapping functions with IL-13.
STAT6 plays an important role in mediating the immune regulatory signal of IL-4.
The IL-4 gene, along with IL-3, IL-5, IL-13, and CSF2, forms a cytokine gene cluster on chromosome 5q.
IL-4 is located near IL-13 and IL-5, regulated by long-range elements over a 120 kb range.
IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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