Thermo Fisher Scientific IRS1 Recombinant Superclonal Antibody (21HCLC)

Applications

Western Blot (WB)

• Tested Dilution: 4–5 µg/mL

Product Specifications

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are composed of multiple recombinant monoclonal antibodies, combining the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies, and providing consistent performance through recombinant production.

Functionally similar to polyclonal antibodies—recognizing multiple epitope sites and enabling higher detection sensitivity for low-abundance targets—these Superclonal™ antibodies have a defined mixture of light and heavy chains, ensuring reproducibility across lots and eliminating biological variability.

Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and performance remain unchanged.

Target Information

IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to stimulation by insulin, insulin-like growth factor 1 (IGF-1), and interleukin 4 (IL-4). It undergoes serine, threonine, and tyrosine phosphorylation across various tissues.

Casein kinase II mediates part of the insulin-stimulated serine/threonine phosphorylation of IRS-1 in vivo, with Thr502 and Ser99 identified as major phosphorylation sites. This phosphorylation may be a component of the intracellular insulin signaling cascade.

IRS-1 also contains three putative binding sites for 14-3-3 (Ser270, Ser374, and Ser641), with the Ser270 motif located in the phosphotyrosine binding domain responsible for interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.