Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor and stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells, mast cells, and plays a role in their proliferation and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse bone marrow cells; ≤0.25 µg/test
• Excitation: 350 nm
• Emission: 395 nm
• Laser: Ultraviolet Laser

Brilliant Ultra Violet™ 395 (BUV395) is designed for use on cytometers equipped with a 355 nm UV laser.
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions when using multiple polymer dye-conjugated antibodies.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (MGF, stem cell factor). KIT regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.

Upon ligand binding, c-Kit dimerizes and autophosphorylates tyrosine residues, activating downstream signaling pathways such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT. c-Kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures.

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