Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Ultra Violet 395, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: Assay-dependent

Control (Ctrl)

• Tested Dilution: Assay-dependent

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Functional Grade
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

• Description: Monoclonal rat IgG1 kappa is useful as an isotype control immunoglobulin.
• Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
• Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.
• Fluorochrome Information: Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for use on cytometers equipped with a 355 nm ultraviolet laser. Ensure your instrument can detect this fluorochrome.
• Staining Buffer Recommendation: When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
• Excitation / Emission / Laser: 350 nm / 395 nm / Ultraviolet Laser.
• Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies commonly used in research and diagnostics.
These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants.
Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens.
It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens.
Gene targeting in rats enables manipulation of specific genes to study human disease mechanisms.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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