Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 395, eBioscience
인간 CD123 (IL-3 수용체 α사슬)을 인식하는 6H6 모노클론 항체로, Brilliant Ultra Violet 395 형광체가 결합된 제품입니다. 유세포 분석용으로 최적화되어 있으며, 사전 희석되어 바로 사용 가능. 4°C에서 어둡게 보관하며 동결 금지.
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Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 395, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Ultra Violet™ 395 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H6 |
| Conjugate | Brilliant Ultra Violet™ 395 |
| Excitation / Emission Max | 347 / 399 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929036 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 480
- Brilliant Violet 786
- eFluor 450
- FITC
- NovaFluor Blue 510
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60–70 kDa transmembrane protein binds IL-3 with low affinity alone and with high affinity when associated with CD131 (common beta chain). CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.125 µg) per test
- Recommended cell range: 10⁵–10⁸ cells/test
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is designed for use on cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family. It forms a heterodimer with the common beta (CDw131) subunit to transmit IL-3 signals. IL-3Ra is overexpressed in acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells, making it a potential therapeutic target.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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