Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 660
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 800
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 690
• NovaFluor Yellow 810
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitory receptor of the C-type lectin superfamily. It contains an ITIM domain and is expressed on subsets of gamma-delta T cells, NK (CD56dim), CD8+, and CD4+ T cells.
KLRG1 is primarily expressed by effector/memory phenotype cells that are short-lived but capable of immediate effector function. Cadherin-E, -N, and -R are ligands for KLRG1, and their interaction inhibits cytolytic activity and proliferation.
The percentage of KLRG1 positive cells can vary depending on antigen experience. Clones 13F12F2 and 13A2 recognize a similar epitope based on cross-blocking studies.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use at 5 µL (1.0 µg) per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for use on cytometers equipped with a 355 nm ultraviolet laser.
Ensure your instrument can detect this fluorochrome.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

SIGLEC5 is a putative adhesion molecule mediating sialic-acid dependent binding to cells. It binds both α2,3- and α2,6-linked sialic acids. The recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Expressed by monocytic/myeloid lineage cells, SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, and bone marrow, and lower levels in lymph node, lung, appendix, placenta, pancreas, and thymus.
It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early myelomonocytic differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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