Thermo Fisher Scientific IL-32A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1:20,000–1:100,000

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid.
• Dilute only prior to immediate use.
• In ELISA and other immunoreactive assays, recognizes both native and recombinant human IL-32A in cell supernatants and certain body fluids.
• A control of similarly diluted normal rabbit IgG is recommended.

Target Information

Interleukin-32 (IL-32) was initially identified as a transcript (NK4) selectively expressed in lymphocytes and NK cells, with expression increased following IL-2 activation. It was later re-isolated from an IL-18-treated lung carcinoma cell line and renamed IL-32.
IL-32 is unique in lacking sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four spliced isoforms.
Treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines (TNF-α, IL-8, MIP-2). It is also induced in human peripheral lymphocytes after mitogen stimulation and in epithelial cells by IFN-γ, suggesting a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.