Thermo Fisher Scientific 4-1BB Ligand Polyclonal Antibody, PeproTech

Thermo Fisher Scientific 4-1BB Ligand Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
MREGPELSPD DPAGLLDLRQ GMFAQLVAQN VLLIDGPLSW YSDPGLAGVS LTGGLSYKED TKELVVAKAG VYYVFFQLEL RRVVAGEGSG SVSLALHLQP LRSAAGAAAL ALTVDLPPAS SEARNSAFGF QGRLLHLSAG QRLGVHLHTE ARARHAWQLT QGATVLGLFR VTPEIPAGLP SPRSE

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human 4-1BB Ligand. Anti-Human 4-1BB Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human 4-1BB Ligand matrix.

Sandwich ELISA:
To detect Human 4-1BB Ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human 4-1BB Ligand (500-P169Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human 4-1BB Ligand.

Western Blot:
To detect Human 4-1BB Ligand by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human 4-1BB Ligand is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CD137 ligand (CD137L) is a type II membrane protein belonging to the TNF superfamily. It functions as a co-stimulatory molecule expressed on antigen-presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on various cells including inflamed endothelial cells, enhancing extravasation of CD137L-expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is expressed in many B-cell lymphomas and carcinoma cell lines, playing a role in T cell–tumor cell interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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