Thermo Fisher Scientific 4-1BB Ligand Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific 4-1BB Ligand Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MREGPELSPD DPAGLLDLRQ GMFAQLVAQN VLLIDGPLSW YSDPGLAGVS LTGGLSYKED TKELVVAKAG VYYVFFQLEL RRVVAGEGSG SVSLALHLQP LRSAAGAAAL ALTVDLPPAS SEARNSAFGF QGRLLHLSAG QRLGVHLHTE ARARHAWQLT QGATVLGLFR VTPEIPAGLP SPRSE.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human 4-1BB Ligand. Anti-Human 4-1BB Ligand-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect h4-1BBL by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human 4-1BBL (500-P169) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant h4-1BBL.

Western Blot:
To detect h4-1BBL by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant h4-1BBL is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P169BT-1MG will be provided as 2 × 500 µg.

Target Information

CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. It is a co-stimulatory molecule expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is expressed on most B-cell lymphomas (mantle, follicular, diffuse large B-cell lymphoma) but not on Hodgkin or T-cell lymphoma. It is also expressed in carcinoma cell lines and is thought to be involved in T cell–tumor cell interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.