Thermo Fisher Scientific Phospho-NGEF (Tyr87) Polyclonal Antibody
Phospho-NGEF (Tyr87) 폴리클로날 항체로 Western blot 및 Immunocytochemistry에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 Rabbit IgG 항체. PBS/BSA/glycerol 완충액에 보관하며 -20°C에서 안정적 유지.
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- PA5143697
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-ephexin-1 (Tyr-87) synthetic peptide (coupled to carrier protein), corresponds to amino acids surrounding tyrosine 87 in mouse ephexin-1. This sequence has significant homology to the conserved region in human and rat ephexin-1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942925 |
Product Specific Information
This antibody detects an 80 kDa band corresponding to ephexin-1 in Western blots of human A431 treated with pervanadate. The band is removed by alkaline phosphatase treatment, confirming phospho-specificity.
Target Information
NGEF acts as a guanine nucleotide exchange factor (GEF) that differentially activates the GTPases RHOA, RAC1, and CDC42. It plays a role in axon guidance by regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity shifts toward RHOA activation, promoting cone retraction while reducing RAC1- and CDC42-mediated growth cone extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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