Thermo Fisher Scientific Phospho-RAD17 (Ser656) Polyclonal Antibody
RAD17 단백질의 Ser656 인산화 형태를 인식하는 토끼 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 높은 특이성과 재현성을 가지며, 세포 성장 및 DNA 손상 반응 연구에 적합. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-RAD17 (Ser656) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Rad17 (phospho Ser656) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792852 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control: 293T (100 µM etoposide treatment for 2 hrs).
- Centrifuge briefly prior to opening the vial.
Target Information
Rad17 is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage.
It has weak ATPase activity required for chromatin binding and participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, as well as CHEK1 activation.
Rad17 may also serve as a sensor of DNA replication progression and be involved in homologous recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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