Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Super Bright 780, eBioscience

Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Super Bright 780, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 6 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV/Violet series (395–805)
• eFluor 450, FITC
• NovaFluor Blue/Red/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702
• Custom conjugation available

Product Specific Information

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells, and is upregulated upon T-cell stimulation. CD27 binds to CD70 and plays an important role in T cell–B cell interaction.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested for flow cytometry of normal human peripheral blood cells. Recommended at 5 µL (0.25 µg) per test (100 µL final volume).

Super Bright 780 is a tandem dye excited by the violet laser (405 nm) and emits at 780 nm. Recommended filter: 780/60 bandpass. Ensure instrument compatibility.

Staining Panels:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Compensation:
Higher compensation values may occur in the violet 450/50 channel when using UltraComp eBeads (Product # 01-2222-42) compared to single-color stained cells. In such cases, set compensation using cells. Similar observations with AbC Total Antibody Compensation beads (Product # A10497).

Light Sensitivity: Protect vial and samples from light to prevent photo-induced oxidation.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 405 nm
Emission: 780 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily, including CD40 and CD30, which regulate cell proliferation and death. CD27 and its ligand CD70 are mainly expressed on lymphocytes. CD27 forms a disulfide-linked homodimer on mature thymocytes, peripheral T cells, and some B cells.

Activation of T cells via TCR-CD3 complex upregulates CD27 expression and releases soluble CD27 (sCD27), a prognostic marker for B cell malignancies. CD27-binding protein (SIVA) mediates apoptosis through CD27. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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