Thermo Fisher Scientific CD36 Monoclonal Antibody (HM36), Super Bright 780, eBioscience

Thermo Fisher Scientific CD36 Monoclonal Antibody (HM36), Super Bright 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.5 µg/test
• View publication

Product Specifications

Additional Conjugate Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• PE
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The HM36 antibody recognizes mouse CD36 (scavenger receptor BIII).

Applications Reported:
Used for flow cytometric analysis.

Applications Tested:
Validated in flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. Recommended ≤0.5 µg/test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Optimize antibody concentration for best assay performance.

Staining Recommendations:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) to reduce non-specific polymer interactions.

Compensation Notes:
Higher compensation values may occur with UltraComp eBeads microspheres (Product # 01-2222-42) compared to single-color stained cells. Set compensation using cells when possible. Similar observations with AbC Total Antibody Compensation beads (Product # A10497).

Light Sensitivity:
Protect vial and stained samples from light; dye is sensitive to photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222)
• 1-step Fix/Lyse Solution (Product # 00-5333)

Excitation/Emission:
405 nm excitation / 780 nm emission (Violet Laser).

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD36 (scavenger receptor class B member 3) is expressed on macrophages, platelets, and adipocytes. It plays roles in lipid metabolism, inflammation, and atherosclerosis. CD36 binds oxidized LDL, long-chain fatty acids, apoptotic cells, collagen, and thrombospondin. It mediates cytoadherence of Plasmodium falciparum-infected erythrocytes. Mutations cause platelet glycoprotein deficiency. Associated diseases include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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