Thermo Fisher Scientific TRAP220 Polyclonal Antibody
TRAP220 단백질을 인식하는 Rabbit Polyclonal 항체로, Human 및 Mouse 시료에 반응합니다. Western blot, ICC/IF, ChIP assay에 적합하며, 고순도(>95%) 항원 친화 정제 제품입니다. PBS/glycerol buffer에 보관되며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 2.5 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1420–1470 of human TRAP220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553376 |
Product Specific Information
- Detects endogenous TRAP220 protein at a molecular weight of approximately 220 kDa.
- Purity >95% as determined by SDS-PAGE.
Target Information
TRAP220 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge between gene-specific regulatory proteins and the basal RNA polymerase II transcription machinery. It is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and general transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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