Thermo Fisher Scientific TRAP220 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

ChIP assay (ChIP)

2.5 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 1420-1470 of human TRAP220 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRAP220,uniProtId:Q15648-1,ncbiNodeId:9606,antigenRange:1420-1470,antigenLength:1581,antigenImageFileName:PA5-36114_TRAP220_Q15648-1_Rabbit.svg,antigenImageFileNamePDP:PA5-36114_TRAP220_Q15648-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553376

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 220 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.