Thermo Fisher Scientific ERBB4 Monoclonal Antibody (OTI1H6), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine kinase subfamily, which includes EGFR, ERBB2, and ERBB3.
ERBB4 binds and is activated by neuregulins and other factors, inducing cellular responses such as mitogenesis and differentiation.

• Activated by neuregulins and NTAK (neural and thymus-derived activator for ErbB kinases)
• Receptor for heregulin, mediating HGL-stimulated tyrosine phosphorylation
• Regulates key cellular processes including metabolism, transcription, cell cycle, cytoskeletal rearrangement, apoptosis, and differentiation

Expression:
Highly expressed in several breast carcinoma cell lines and normal tissues such as skeletal muscle, heart, pituitary, brain, and cerebellum.
High mRNA levels: T47-D, MDA-MB-453, BT-474, H3396
Intermediate levels: MCF-7, MDA-MB-330, MDA-MB-361
Low or absent: MDA-MB-231, MDA-MB-157, MDA-MB-468, SKBR-3

Elevated ERBB4 levels have been observed in certain tumor cell lines, suggesting a possible role in malignancy.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.