Thermo Fisher Scientific EEA1 Polyclonal Antibody
EEA1 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 등 반응성. Protein A로 정제된 1 mg/mL 농도의 액상 시약으로, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human EEA1, amino acids 1251–1350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to early endosomes and essential for the fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments that mediate redistribution of extracellular compounds. EEA1 contains zinc-finger-like domains in both amino and carboxyl-terminal regions, with the carboxyl-terminal domain being a conserved zinc-binding region important for intracellular localization. Membrane association of EEA1 depends on phosphatidyl 3-kinase activity; inhibitors of this enzyme cause EEA1 to dissociate from early endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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