Thermo Fisher Scientific Phospho-AP2M1 (Thr156) Polyclonal Antibody
인간, 마우스, 랫트 반응성을 가진 Rabbit Polyclonal 항체로서 AP2M1 Thr156 인산화 부위를 인식합니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합하며, 고순도 Protein A 정제 및 안정한 TBS/glycerol 버퍼로 제공됩니다.
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Thermo Fisher Scientific Phospho-AP2M1 (Thr156) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human AP2M1 around the phosphorylation site of Thr156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase responsible for proton pumping during endosome and lysosome acidification.
AP2M1 associates with the HIV-1 protein Nef, suggesting Nef may use the AP-2 complex to enhance endocytosis of CD4 and class I MHC. It may also regulate intracellular trafficking and function of CTLA-4 protein. At least two isoforms of AP2M1 are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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