Thermo Fisher Scientific Lamin A/C Monoclonal Antibody (mab636)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

View 11 publications 11 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human, Mouse, Pig

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

mab636

Immunogen

Porcine lamin preparation. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Lamin A/C,uniProtId:Q3ZD69-1,ncbiNodeId:9821,antigenRange:1-664,antigenLength:664,antigenImageFileName:MA3-1000_Lamin_AC_Q3ZD69-1_House_mouse.svg,antigenImageFileNamePDP:MA3-1000_Lamin_AC_Q3ZD69-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325377

Product Specific Information

MA3-1000 detects lamin A/C from human, bovine, porcine and canine samples.

MA3-1000 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects ~ 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract. This antibody can also be used for immunofluorescence and immunohistochemistry for subcellular localization experiments. MA3-1000 has been widely used for detection of lamin A/C control in siRNA experiments.

The MA3-1000 antigen is porcine lamin preparation.

Target Information

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.