Thermo Fisher Scientific Phospho-FOXO1 (Ser329) Polyclonal Antibody
FOXO1 단백질의 Ser329 인산화 상태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 액상형, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human FOXO1 (Accession Q12778), corresponding to amino acid residues around phosphorylated Ser329. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816449 |
Product Specific Information
This antibody detects endogenous levels of FOXO1A only when phosphorylated at Serine 329.
Target Information
FOXO1 (FKHR, Forkhead Box O1) is a member of the forkhead homeotic gene family of transcription factors. It can function as a coactivator or corepressor of nuclear receptor activity through the LXXLL motif in its C-terminal region. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. FOXO1 plays roles in autophagy, myogenic differentiation, and acts as a key regulator in insulin signaling and metabolic homeostasis. It binds to insulin response elements (IRE) and Daf-16 family binding elements (DBE) to regulate gene expression. FOXO1 influences redox balance, osteoblast differentiation, bone mass, and glucose metabolism by modulating RUNX2 activity and promoting gluconeogenesis in hepatocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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