Thermo Fisher Scientific BUB1B Monoclonal Antibody (OTI10E7), TrueMAB
인간 BUB1B 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC/IF에 사용 가능. Lyophilized 형태로 제공되며, PBS 및 trehalose buffer에 보존. 세포 분열 체크포인트 연구 및 암 관련 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10E7 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human BUB1B expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
BUBR1 is a Ser/Thr protein kinase involved in the mitotic checkpoint, delaying anaphase until chromosomes are properly attached to the spindle. Defects in this checkpoint contribute to genetic instability and cancer development.
Human cells contain two checkpoint kinases derived from the BUB1 gene: hBUBR1 and hBUB1. Both regulate kinetochore-microtubule interactions during mitosis.
BUBR1 is essential for proper mitotic progression and has been detected in various human cancers. It interacts with BCSG1, potentially accelerating breast cancer progression by compromising checkpoint control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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