Thermo Fisher Scientific BUB1B Monoclonal Antibody (OTI3D9), TrueMAB
인간 BUB1B 단백질에 특이적인 Mouse IgG2b 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. TrueMAB 형식으로 정제된 고순도 항체이며, 재구성 및 결합 실험에 적합. 연구용으로만 사용 가능.
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Thermo Fisher Scientific BUB1B Monoclonal Antibody (OTI3D9), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D9 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human BUB1B expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
BUBR1 (BUB1B) is a Ser/Thr protein kinase involved in the mitotic checkpoint, delaying anaphase until chromosomes are properly attached to the mitotic spindle. Defects in this checkpoint contribute to genetic instability and cancer progression.
Human cells express two related checkpoint kinases, hBUBR1 and hBUB1, which regulate kinetochore-microtubule interactions during mitosis.
BUBR1 is essential for normal mitotic progression, preventing premature anaphase entry, and has been identified as a binding partner of BCSG1, implicated in breast cancer progression through checkpoint inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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