Thermo Fisher Scientific Phospho-CD247 (CD3 zeta) (Lys142) Monoclonal Antibody (EM-54), Alexa Fluor 488

Applications

• Flow Cytometry (Flow): 1–5 µg/mL

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 647
• Request custom conjugation

Product Specific Information

This antibody recognizes phosphorylated intracellular tyrosine 142 of CD3 zeta chain (CD247), which is a component of the TCR/CD3 complex expressed on T cells.

Target Information

The CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon, and CD3 zeta. These subunits are structurally related members of the immunoglobulin superfamily encoded by closely linked genes on human chromosome 11.

CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules through a double tyrosine-based motif. CD3 may play a role in TCR-induced growth arrest, cell survival, and proliferation. The CD3 antigen is present on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.

Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency due to CD3delta/CD3epsilon/CD3zeta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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