Thermo Fisher Scientific Acetyl-CoA Carboxylase Recombinant Rabbit Monoclonal Antibody (5J4W7)
Acetyl-CoA Carboxylase 단백질을 검출하기 위한 Recombinant Rabbit Monoclonal 항체. Western blot, IHC, ELISA, IP에 사용 가능. 높은 특이성과 재현성을 제공하며 다양한 종(Human, Mouse, Rat)에 반응. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5J4W7 |
| Immunogen | Synthetic peptide corresponding to amino acids 350–450 of human ACC1 (Q13085) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911858 |
Product Specific Information
Positive test controls include:
- HeLa
- HepG2
- C6
- Mouse liver
- Mouse heart
Immunogen sequence:
RLAKQSRHLE VQILADQYGN AISLFGRDCS VQRRHQKIIE EAPATIATPA VFEHMEQCAV KLAKMVGYVS AGTVEYLYSQ DGSFYFLELN PRLQVEHPCTE
Target Information
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by distinct genes. ACC-alpha is highly enriched in lipogenic tissues.
The enzyme is regulated both long term (transcriptional and translational levels) and short term (phosphorylation/dephosphorylation of serine residues, allosteric transformation by citrate or palmitoyl-CoA). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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