Thermo Fisher Scientific 6x-His Tag Chimeric Recombinant Mouse Monoclonal Antibody (3D5)
6x-His 태그 단백질 검출용 마우스 재조합 단일클론 항체. Western blot, Flow cytometry, ELISA 등 다양한 어플리케이션에 사용 가능. C-말단 및 내부 His-tag에 결합하며, N-말단 His-tag에는 반응하지 않음. Protein A 정제, 1 mg/mL 농도, PBS 버퍼에 ProClin 300 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG2c, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3D5 |
| Immunogen | Purified His tagged Fab fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins.
It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at the N- or C-terminal region of recombinant proteins to facilitate purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection or purification of recombinant proteins by fusing a known epitope sequence.
His-tagged proteins can be purified using Ni²⁺-NTA affinity chromatography due to the strong and selective chelation between histidine residues and nickel ions.
His tag-specific antibodies are widely used for detection, co-immunoprecipitation, and validation of tagged recombinant proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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