Thermo Fisher Scientific NF-H Monoclonal Antibody (RNF 402)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25-1:250

-

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat, Xenopus

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

RNF 402

Immunogen

Neurofilament preparation of calf brain tissue

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325537

Product Specific Information

OMA1-06116 detects both the phosphorylated and non-phosphorylated forms of the 200 kDa neurofilament from rat, guinea pig, rabbit, dog, hamster, chicken, mouse, cow, monkey, sheep, xenopus and human tissues.

OMA1-06116 has been successfully used in immunohistochemical (frozen & paraffin) and Western blot procedures. By Western blot, this antibody detects a single ~200 kDa protein representing both forms of the 200 kDa neurofilament from rat brain homogenate.

OMA1-06116 antigen is cytoskeletal preparation of calf brain tissue.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.