Thermo Fisher Scientific NEFM Monoclonal Antibody (RNF 406)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:100

-

Product Specifications

Species Reactivity

Bovine, Guinea pig, Hamster, Human, Non-human primate, Rabbit, Rat, Xenopus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RNF 406

Immunogen

Neurofilament preparation of calf brain tissue if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEFM,uniProtId:O77788-1,ncbiNodeId:9913,antigenRange:1-926,antigenLength:926,antigenImageFileName:OMA1-06111_NEFM_O77788-1_House_mouse.svg,antigenImageFileNamePDP:OMA1-06111_NEFM_O77788-1_House_mouse_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325535

Product Specific Information

OMA1-06111 specifically detects the phosphorylated 160 kDa neurofilament from human, guinea pig, hamster, non-human primate, rabbit and xenopus tissues.

OMA1-06111 has been successfully used in Western blot and immunohistochemistry (frozen) procedures. By Western blot, this antibody detects an ~160 kDa protein representing the phosphorylated form of the 160 kDa neurofilament in human brain extract.

OMA1-06111 antigen is cytoskeletal preparation of calf brain tissue.

Target Information

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.