Thermo Fisher Scientific PRMT5 Monoclonal Antibody (OTI1F8), TrueMAB
PRMT5 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot에 최적화되어 있습니다. Human 유래 항원(aa 238-499)을 기반으로 제작되었으며, Lyophilized 형태로 제공됩니다. 재조합 단백질 연구 및 단백질 메틸화 연구에 적합합니다.
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Applications
- Western Blot (WB): 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 238-499 of human PRMT5 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that catalyzes the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for MMA formation.
It specifically mediates symmetrical dimethylation of arginine residues in small nuclear ribonucleoproteins Sm D1 and Sm D3, a process essential for snRNP core particle assembly and biogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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