Thermo Fisher Scientific Estrogen Receptor Alpha Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen hormone, undergoes a conformational change enabling dimerization and binding to estrogen response elements (ERE) in DNA.
When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins.

There are two forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2). Due to alternative RNA splicing, at least four estrogen receptor-alpha isoforms are known:

• Isoform 1 (66 kDa)
• Isoform 2 (53 kDa)
• Isoform 3 (47 kDa)
• Isoform 4 (35 kDa)

Estrogen receptors are widely expressed in various tissues and are essential for sexual development and reproductive function. They also play roles in other tissues such as bone and are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.