Thermo Fisher Scientific Estrogen Receptor Alpha Polyclonal Antibody
에스트로겐 수용체 알파(ERα)를 인식하는 Thermo Fisher Scientific의 폴리클로날 항체. Western blot, IHC, IP, ChIP-Seq 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 인간에 반응하며 마우스와 랫에서도 예측 반응. 연구용 한정.
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Estrogen Receptor Alpha Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | Assay-dependent |
| ChIP-sequencing (ChIP-Seq) | 4 µg / 30 µg chromatin |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 1 and 50 of human Estrogen Receptor Alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen hormone, undergoes a conformational change enabling dimerization and binding to estrogen response elements (ERE) in DNA.
When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins.
There are two forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2). Due to alternative RNA splicing, at least four estrogen receptor-alpha isoforms are known:
- Isoform 1 (66 kDa)
- Isoform 2 (53 kDa)
- Isoform 3 (47 kDa)
- Isoform 4 (35 kDa)
Estrogen receptors are widely expressed in various tissues and are essential for sexual development and reproductive function. They also play roles in other tissues such as bone and are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.