Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody
Phospho-Histone H3 (Ser10) 폴리클로날 항체로, 인간 및 마우스 시료에 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 액상 형태이며, 4°C에서 보관합니다.
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Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:2,000–1:10,000 |
| Flow Cytometry (Flow) | 0.4 µg/1×10⁶ cells in 150 µL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphorylated peptide representing a portion of Human Histone H3 surrounding serine 10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Notes:
- For ICC, formaldehyde fixation and permeabilization with Triton-X 100 is recommended.
- Acetone fixation may adversely affect staining.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat, D. melanogaster, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Rabbit, Pig, and Orangutan.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with four core histone proteins, H3 binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications such as methylation, acetylation, and phosphorylation influence chromatin structure and transcription factor binding. The N-terminal tail of histone H3 is subject to these modifications, affecting various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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