Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:10,000

-

Immunohistochemistry (IHC)

1:2,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000-1:10,000

-

Immunocytochemistry (ICC/IF)

1:2,000-1:10,000

-

Flow Cytometry (Flow)

0.4 µg/1x10^6 cells in 150 µL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphorylated peptide, which represented a portion of Human Histone H3 surrounding serine 10. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Histone H3,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:10,antigenLength:136,antigenImageFileName:A301-844A_Histone_H3_P68431-1_Rabbit.svg,antigenImageFileNamePDP:A301-844A_Histone_H3_P68431-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For ICC, formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Rat, D. melanogaster, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Rabbit, Pig and Orangutan

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.