Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody
Phospho-Histone H3 (Ser10) 폴리클로날 항체로 다양한 종에서 반응 예측. WB, IHC, ICC, Flow 등 폭넓은 응용 가능. 포름알데히드 고정 및 Triton-X 100 처리 권장. 액상 형태, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:2,000–1:10,000 |
| Flow Cytometry (Flow) | 0.4 µg / 1×10⁶ cells in 150 µL |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphorylated peptide representing a portion of Human Histone H3 surrounding serine 10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Notes:
- For ICC: Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely affect staining.
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Predicted Reactivity: Rat, D. melanogaster, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Rabbit, Pig, Orangutan
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, H3 binds to DNA to form nucleosomes, playing a crucial role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histone H3, including methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression. The N-terminal tail of H3 can undergo various modifications affecting cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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