Thermo Fisher Scientific Goat anti-Human IgG Fc Highly Cross-Adsorbed Secondary Antibody, AP
Goat anti-Human IgG Fc 알칼라인 포스파타제(AP) 결합 2차 항체로, Western blot, IHC, ELISA에 적합합니다. 고도로 교차흡착되어 비특이 반응을 최소화하며, 높은 민감도와 신호 증폭 효과를 제공합니다. 연구용으로만 사용 가능합니다.
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- A18832
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 1% BSA, 30 mM triethanolamine, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535609 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins, as well as human IgA and IgM |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Biotin conjugate
- FITC conjugate
- HRP conjugate
- TRITC conjugate
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. These antibodies are useful for detection, sorting, or purification of human immunoglobulins.
Secondary antibodies enhance experimental flexibility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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