Thermo Fisher Scientific PrP Chimeric Recombinant Mouse Monoclonal Antibody (3F4)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.001 µg/mL

-

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Hamster, Human

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3F4

Immunogen

Hamster 263K PrPs. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PrP,uniProtId:Q60506-1,ncbiNodeId:10026,antigenRange:1-254,antigenLength:254,antigenImageFileName:MA5-47985_PrP_Q60506-1_House_mouse.svg,antigenImageFileNamePDP:MA5-47985_PrP_Q60506-1_House_mouse_PDP.jpeg,sortOrder:6}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074428

Target Information

Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.