Thermo Fisher Scientific CCL23 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3 (CCL23). The antibody was purified by affinity chromatography using an immobilized Human MIP-3 (CCL23) matrix.

Sandwich ELISA:
To detect Human MIP-3 (CCL23) by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When paired with PeproTech Biotinylated Anti-Human MIP-3 (CCL23) (500-P124Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human MIP-3 (CCL23).

Western Blot:
For Western Blot analysis, use 0.1–0.2 µg/mL of this antibody. In conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3 (CCL23) is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

This gene is part of a chemokine gene cluster located on the q-arm of chromosome 17. Chemokines are secreted proteins involved in immune regulation and inflammation.
This chemokine, a member of the CC subfamily, exhibits chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils, and no activity on activated T lymphocytes.
It strongly suppresses colony formation by multipotential hematopoietic progenitor cells and acts as a potent agonist of the chemokine (C-C motif) receptor 1.
Alternative splicing generates multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.