Thermo Fisher Scientific CCL23 Polyclonal Antibody, PeproTech
Human CCL23 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot, ELISA, Flow Cytometry에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 완충액에 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Flow Cytometry (Flow) | - | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MIP-3 (CCL23) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929347 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3 (CCL23). The antibody was purified by affinity chromatography using an immobilized Human MIP-3 (CCL23) matrix.
Sandwich ELISA:
To detect Human MIP-3 (CCL23) by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When paired with PeproTech Biotinylated Anti-Human MIP-3 (CCL23) (500-P124Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human MIP-3 (CCL23).
Western Blot:
For Western Blot analysis, use 0.1–0.2 µg/mL of this antibody. In conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3 (CCL23) is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
This gene is part of a chemokine gene cluster located on the q-arm of chromosome 17. Chemokines are secreted proteins involved in immune regulation and inflammation.
This chemokine, a member of the CC subfamily, exhibits chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils, and no activity on activated T lymphocytes.
It strongly suppresses colony formation by multipotential hematopoietic progenitor cells and acts as a potent agonist of the chemokine (C-C motif) receptor 1.
Alternative splicing generates multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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