Thermo Fisher Scientific CCL23 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3 (CCL23). Anti-Human MIP-3 (CCL23)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human MIP-3 (CCL23) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIP-3 (CCL23) (500-P124) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human MIP-3 (CCL23).

Western Blot:
To detect hMIP-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hMIP-3 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P124BT-1MG will be provided as 2 × 500 µg.

Target Information

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide.

This chemokine, a member of the CC subfamily, displays:

• Chemotactic activity on resting T lymphocytes and monocytes
• Lower activity on neutrophils
• No activity on activated T lymphocytes

It is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line and a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.