Thermo Fisher Scientific CCL16 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human LEC (CCL16) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL16,uniProtId:O15467-1,ncbiNodeId:9606,antigenRange:24-120,antigenLength:120,antigenImageFileName:500-P125GBT-500UG_CCL16_O15467-1_Goat.svg,antigenImageFileNamePDP:500-P125GBT-500UG_CCL16_O15467-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human LEC (CCL16). Anti-Human LEC (CCL16)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human LEC (CCL16) (500-P125G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human LEC (CCL16).

Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hLEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P125GBT-1MG will be provided as 2 x 500 µg

Target Information

CCL16 gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. CCL16 displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.