Thermo Fisher Scientific SKA1 Polyclonal Antibody
SKA1 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 분열 및 방추체-키네토코어 복합체 연구용으로 이상적입니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.5–1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 13 amino acid peptide from near the carboxy terminus of human SKA1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155436 |
Product Specific Information
- Suggested positive control: A549 cell lysate
- Blocking peptide: PEP-0931 compatible with PA5-20817
Target Information
Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, enabling chromosome segregation. Kinetochores (KT) on centromeric chromatin are essential for proper chromosome attachment to spindle MTs.
The spindle and kinetochore complex (SKA complex), composed of SKA1, SKA2, and SKA3, is required for stable KT-MT interactions and timely anaphase onset.
Depletion of SKA1 or SKA2 by siRNA removes both proteins from the KT without affecting KT structure. Cells lacking the SKA complex exhibit prolonged metaphase-like delay, underscoring its role in metaphase plate maintenance and spindle checkpoint silencing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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