Thermo Fisher Scientific SKA3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.5–1 µg/mL

Product Specifications

Product Specific Information

• A suggested positive control is human testis tissue lysate.
• PA5-20819 can be used with blocking peptide PEP-0933.
• Antibodies should not be exposed to prolonged high temperatures.

Target Information

Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, allowing segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for proper attachment of chromosomes to spindle MTs.
A spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, is required for stable KT–MT interactions and timely anaphase onset. Depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged metaphase-like state. SKA3-depleted cells accumulate high levels of checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and chromosome cohesion maintenance during mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.